Dulbecco’s Modified Eagle Medium:Nutrient Mixture F-12 (DMEM/F-12) is a widely used basal medium for supporting the growth of many different mammalian cells. Cells successfully cultured in DMEM/F-12 include MDCK, glial cells, fibroblasts, human endothelial cells, and rat fibroblasts.
This DMEM / F-12 is modified as follows:
With
L-glutamine
HEPES
Phenol Red
DMEM/F-12 is a 1:1 mixture of DMEM and Ham’s F-12. This formulation combines DMEM’s high concentrations of glucose, amino acids and vitamins with F-12’s wide variety of components. DMEM/F-12 contains no proteins, lipids, or growth factors. Therefore, DMEM/F-12 may require supplementation, commonly with 10%Fetal Bovine Serum(FBS). DMEM/F-12 uses a sodium bicarbonate buffer system (2.438 g/L) and therefore requires a 5-10% CO2environment to maintain physiological pH.
Gibco ‘BASIC’ products offer value-minded scientists liquid cell culture media and buffer solutions at a significant price savings. As part of Life Technologies’ commitment to quality, this select range of Research Use Only products is continually tested to ensure performance and customer satisfaction.
内容与储存
Storage conditions: 2-8° C. Protect from lightShipping conditions: AmbientShelf life: 12 months from date of manufacture
